Oligo

What are Oligonucleotides?

Oligonucleotides (oligo) are short nucleic polymer chains that bind to DNA and RNA sequences and are used in research, forensics and genetic testing. They are manufactured in a lab by a procedure called solid-phase chemical synthesis.

Uses:

Oligo are used in DNA microarrays, Southern blot assay, ASO analysis, artificial gene synthesis, DNA sequencing, polymerase chain reaction, and fluorescent in situ hybridization (FISH).

Purification of Oligonucleotides:

During the process Oligonucleotides are made, they may accumulate some impurities. This could be a problem in lab procedures which is why Oligo must be purified before they can be used in research. Few ways to purify them are gel filtration, HPLC, reverse HPLC, and anion-exchange HPLC and desalting.

Modification of Oligonucleotides:

Many research projects demand certain modified Oligos to carry out a lab procedure.

The following are some ways to modify Oligos.

• Biotinylation: Biotin is used for DNA and RNA detection. When a biotin is covalently attached to a nucleic acid, it is called Biotinylation.
• Fluorescent labeling (Dyomics™, Cy Dyes: More than 200 labels are available to choose from depending on your research details. Fluorescent labeling is the easier and most widely used method to modify oligos to use in detecting DNA sequences, forensics and such.
• Wobble bases: It is a mixture of bases that can be introduced to DNA at any position in oligo sequence. Such oligonucleotides are usually required for for random mutagenesis, and combinatorial chemistry.
• Phosphorylation: 5' or 3' phosphorylation can be done on oligonucleotides to help in research. 5' phosphorylation is needed when an oligo is used as a DNA ligase substrate. 3' phosphorylation inhibits 3' degeneration and by 3' exonucleases and to block DNA polymerase extension.
• Quenchers: Quenchers are used to absorb energy that is emitted by a fluorophore and while it is absorbing its energy, quenchers inhibits fluorophore's detection. Such modified oligos are important in various types of lab tests.

Off Road Tours in Spain

Adventures in Spain

There are many adventures awaiting the excited traveler right in Spain. There is so much to experience. Off Road Tours in Spain are amazing. There is literally something for every traveler who chooses Spain as their destination. If it is lovely scenes and views that you are looking for then you will be pleasantly surprised to find that the landscape does indeed vary greatly. There are numerous beaches that truly glitter. The deepest and most vibrant waters await you.

Off Road Tours in Spain

You may truly enjoy a guided off road tour. There are many package deals that will offer grand specials for those off road tours. You will not miss out on a single attraction or any of those extra special spots. The following is a sample list of some of the tours that are available to the excited traveler:

• Adventure tours
• Off road tours
• Motorbike tours
• Walking Tours

Off Road Tours in Spain that will lead you through the magnificent and splendid beauty. There are many islands that are waiting for gracious travelers. You may have to take in the grandest bull fight when your in Spain.

Attractions

Spain is filled with wonderous attractions. There are 3 absolutely top attractions that should never be missed. The top 3:

1. Pamplona is a city in Navarra. It is the host of running of the bulls.
2. Palacio Real. This is the Royal Palace of Madrid.
3. Aqueduct of Segovia. This is one of the preserved monuments. The Romans left this beauty.

Off Road Tours in Spain will show you many more unbelievable attractions.

Catalonia

Off Road Tours in Spain will show off the breathtaking Catalonia. You may feel as if you are having a tour of a real fairytale land.

Pita Bread Making Machine

Pita Bread Making Machine

When you are running your own bakery, you want to make sure that you have all the machinery that is needed to manage the business properly. You need to have machines that make a number of different types of bread and pastries, and a pita bread making machine can run all day long as you manage your bakery. Consider the best way to buy one of these machines for your business.

The Speed

You need to get a pita bread making machine that is going to move at the speed you desire. The speed of the pita bread making machine alone is something that determines how efficient your business is. If you cannot keep up with demand, you will not be able to keep your customers happy. Choose the pita bread making machine that is going to help you keep your customers fed all throughout the day.

The Size

Your bakery may be in one of the smallest rooms you could imagine. If this is the case, you need to have a pita bread making machine that is going to fit in the room. Also, you need to be sure that the smallest machine will go at the speed you need. If you are trying to produce as much bread as possible, you should get a pita bread making machine that is going to be big enough to meet demand.

The PricingYou must find a pita bread making machine that is going to fit into you budget. The budget for your business is likely set in stone. You must weigh the speed of the machine with the size of the machine and its price. When you have considered all these steps, you will be able to find a machine that is going to keep your bakery humming all day.

Day Tours from Barcelona

Discover all there is to offer with Day Tours from Barcelona! Spend the entire day while taking your time to explore all there is on a walking day tour from Barcelona where you can take in the Plaça de Sant Jaume where local and regional government affairs take place. Then take a walk over to the Plaça del Rei. Just to get you started. Wireless tours will be led by professional tour guides allowing for communication between you and the tour guide. Walking Day tours from Barcelona last approximately 2 hours, giving you plenty of time to take in other sites.

If you are a fan of the arts then be sure to make use of the Articket as another choice of Day Tours from Barcelona. This ticket will gain entry to Museu Picasso, Fundació Joan Miró, Museu Nacional d'Art de Catalunya- MNAC, Centre de Cultura Contemporània de Barcelona - CCCB. Along with Fundació Antoni Tàpies and Museu d'Art Contemporani de Barcelona - MACBA. All of which will satisfy your appetite for any and all things of historic Barcelona art.

If you are feeling a little more adventurous, eBikes offer an excellent method of travel for another option to Day Tours from Barcelona. Electric eBikes will give you 60-100km of travel on a single charge! Don't worry, we won't send you out without GPS guidance from your smartphone, a helmet, lock, water bottle and a map of the city. Feel confident as you explore everything from the sea to the mountains.

If wholesome food is as essential to your travel experience as it is mine, you will want to take in the walking gourmet food Day Tours from Barcelona. Tour the top restaurants in the city, sure to find pleasing to any pallet. Indulge yourself in everything from Tapas, Chocolate, Sweet and of course the Boqueria Market. No matter what your desires are on your adventure, rest assured there are plenty of Day Tours from Barcelona to make you wanting to return soon.

Dead Sea Skin Care

Located in the Middle East, the Dead Sea is the lowest place on Earth. Rich in minerals, the Dead Sea and the black mud at its bottom are excellent for the skin. Dead Sea skin care has been popular since Cleopatra.

 

Dead Sea Waters

Research about the Dead Sea was started in 1778, and is still ongoing. The Dead Sea is 417 meters under the sea level, and is full of salt and minerals. The waters of the Dead Sea contain a concentration of 33% salt and minerals, compared to only 3% in the ocean.

 

 

Minerals

The water of the Dead Sea has a unique mix of highly concentrated salt and minerals. The minerals found in the water include boron, bromine, strontium, zinc, potassium, manganese, calcium, magnesium, lithium, sulfur, sodium, and phosphorus. Even a swim in the waters is considered a form of Dead Sea skin care.

 

Black Mud

The black mud at the bottom of the Dead Sea is another preferred method of Dead Sea skin care. The mud is good for pain relief, such as joint and muscle pain. It also has antibacterial properties.

 

Healing Properties

While Dead Sea skin care may be the most popular association with the Dead Sea, there are other benefits to be discovered. Research has shown that exposure to the Dead Sea has a medicinal effect, with benefits to ailments such as heart disease, lung disease, skin diseases, chronic eye diseases, and rheumatic diseases.

 

Dead Sea Skin Care

Dead Sea skin care products are highly sought-after. From pain relieving sprays and creams, to rejuvenating hand and foot creams, to foot peeling creams and foot deodorant creams, the Dead Sea has allowed for the creation of many useful products.

Tunnel Oven

FLAT PITA BREAD TUNNEL OVEN

QUALITY

Our line of Flat Pita Bread production systems surpasses industry standards for reliability and production rate. With over 50 years of experience, our team of professionals engineered the Flat Pita Bread Tunnel Oven to meet the needs of the Pita Industry, while maintaining top of the line equipment. Our conveyor style Ovens have manual and fully automated styles to suit the needs of your Bakery. The Tunnel Oven can be summed up with few simple words: Low maintenance, high yield, quality customer service, low cost to production value. High quality engineering along with quality testing has proven our Pita Tunnel Oven design to be the number one choice for production facilities around the world.

PRODUCTION RATE

The Pita Bread Tunnel Oven is a high temperature system designed to produce 16000 Pitas per hour, while operating in smaller spaces due to the engineering of the Tunnel Ovens vertical design. The easy to use digital platform ensures that you are spending less time on training and more time on production line. The conveyor style production equipment allows for a steady flow of Pita production, while minimizing the floor space required in your facility.

CUSTOMER SERVICE

Our team of experts are standing by to provide accurate and detailed information on our Flat Pita Bread Tunnel Ovens. Let our team of trained professionals guide you through the operations of the Tunnel Oven with accurate and detailed information, ensuring that you receive the quality and care that you deserve. Please contact us with your comments, questions, or concerns about our Flat Pita Bread Baking Equipment. You can contact us through email, or by telephone, which is located under the contacts page on our website. Thank you for choosing the leading manufacturer of Flat Pita Bread Baking equipment .

Pita Bread Machines

Pita Bread

Long considered a staple of the Mediterranean and Middle Eastern diet, pita bread is continuing to gain popularity with many different food lovers and menus around the world. It is a lightly leavened bread, round flat bread, which is chewy and firm enough to take any filling without becoming soggy. Because of the relatively high temperature these breads are baked at, the inside forms a natural hollow which has made the use of "pita pockets" a handy substitute for messy sandwiches and burritos. Restaurants, cafes and bakeries have doubled and tripled orders for pitas in recent years.

Making Pita Bread

To take full advantage of this rising popularity, you need the right equipment to make bulk orders as efficiently as possible. A baker in a traditional kitchen will only produce a few dozen every hour. Fully automated Pita Bread Machines will produce 16,000 every hour. Each loaf will be the same texture and size to ensure consistent results every time. Since the dough is portioned equally, baking time will be the same for all loaves to keep production at an even speed and on schedule.

Pita Bread Machines

Good quality Pita Bread Machines should be purchased from a manufacturer with experience in selling and repairing bulk-baking equipment. Even the best Pita Bread Machines will need regular maintenance. Ask about warranties and insurance for your new investment. Get a schedule of recommended inspections and production expectations for Pita Bread Machines in general and for major parts.

Customer Service

Before using Pita Bread Machines for the first time, ask for a short demonstration to use the machine properly. Keep the manufacturer's customer service and product repair numbers on file. Ask about estimates for on-site repair. Ensure there is a way to purchase individual replacement pieces, as they are needed 
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